William Twining Jr. was born about 1625 in England. He signed a will on 26 Jun 1697 in Newtown, Bucks Co., Pennsylvania. He died on 4 Jan 1703 in Newtown, Bucks Co., Pennsylvania. He was a Quaker. He was married to Elizabeth Deane about 1650 in Eastham, Barnstable Co., Massachusetts.

Elizabeth Deane was born in 1630 in Plymouth Colony. She died on 28 Feb 1709 in Newtown, Bucks Co., Pennsylvania. Cutter has 28 Dec 1708. Children were:

i. Elizabeth Twining.
ii. Ann Twining was born about 1652.
iii. William Twining was born on 28 Feb 1653/54 in Eastham, Barnstable Co., Massachusetts. He died on 23 Jan 1734/35 in Eastham, Barnstable Co., Massachusetts.
iv. Susannah Twining was born on 25 Jan 1654/55 in Eastham, Barnstable Co., Massachusetts. "Cape Cod Library..." and Cutter have 25 Feb. 1654.
v. Joanna Twining was born on 30 May 1657 in Eastham, Barnstable Co., Massachusetts.
vi. Stephen Twining was born on 6 Feb 1659/60 in Eastham, Barnstable Co., Massachusetts.
vii. Mehitable Twining was born in 1660 in Eastham, Barnstable Co., Massachusetts.