Maj. Samuel Appleton Jr. was born about 1624/25 in Little Waldingfield, co. Suffolk, England. Hammatt has a birth date of about 1626. He was baptized on 2 Feb 1624/25 in Little Waldingfield, co. Suffolk, England. He took the oath of freeman on 26 Sep 1648. He served in the military in 1676. He signed a will on 17 Apr 1695. He died on 15 May 1696 in Ipswich, Essex Co., Massachusetts. His estate was probated on 25 May 1696. He was buried in Old Burying Ground, Ipswich, Essex Co., Massachusetts. He was also known as Samuel Apleton. 

He married first Hannah Paine (daughter of  William and Hannah Paine) on 2 Apr 1651 in Ipswich, Essex Co., Massachusetts.

He married second Mary Oliver on 8 Dec 1656 in Newbury, Essex Co., Massachusetts. Hammatt says 2 Dec. 1656.

Mary Oliver was born on 7 Jun 1640 in Newbury, Essex Co., Massachusetts. She died on 15 Feb 1697/98 in Ipswich, Essex Co., Massachusetts. She was buried in Ipswich, Essex Co., Massachusetts. She was also known as Mary Olliver. Children were:

i. John Appleton.
ii. Maj. Isaac Appleton was born in 1664 in Ipswich, Essex Co., Massachusetts. He died on 22 May 1747 in Ipswich, Essex Co., Massachusetts. He was a gentleman.
iii. Joanna Appleton was born in Ipswich, Essex Co., Massachusetts.
iv. Joseph Appleton was born on 5 Jun 1674 in Ipswich, Essex Co., Massachusetts. He died in 1676 in Ipswich, Essex Co., Massachusetts. "Clark and Worth" says he died in 1689.
v. Oliver Appleton was born in Jun 1676 in Ipswich, Essex Co., Massachusetts. He died on 3 Jun 1676 in Ipswich, Essex Co., Massachusetts.
vi. Mary Appleton was born in Jun 1676 in Ipswich, Essex Co., Massachusetts. She died on 9 Jun 1676 in Ipswich, Essex Co., Massachusetts.
vii. Lt. Oliver Appleton was born in 1677 in Ipswich, Essex Co., Massachusetts. He died on 9 Jan 1759 in Ipswich, Essex Co., Massachusetts. He was a yeoman.
viii. Mary Appleton was born on 20 Oct 1679 in Ipswich, Essex Co., Massachusetts. She died in 1689.