Samuel Appleton was born in 1586 in Little Waldingfield, co. Suffolk, England. He was baptized on 13 Aug 1586 in Little Waldingfield, co. Suffolk, England. He emigrated in 1635. He took the oath of freeman on 26 May 1636. He died in Jun 1670 in Rowley, Essex Co., Massachusetts. He was buried in Jun 1670 in Rowley, Essex Co., Massachusetts. He was a gentleman and armiger. He married first Judith Everard on 24 Jan 1615/16 in Preston, co. Suffolk, England.

Judith Everard was born about 1587. She died about 1630. She was also known as Mary Everard. Children of Samuel Appleton and Judith Everard were:

i. Mary Appleton was born in 1616 in Little Waldingfield, co. Suffolk, England. She was baptized on 10 Dec 1616 in Little Waldingfield, co. Suffolk, England. She died young.
ii. Judith Appleton was born in 1618 in Little Waldingfield, co. Suffolk, England. She was baptized on 13 Oct 1618 in Little Waldingfield, co. Suffolk, England. She died in 1629 in Reydon, England.
iii. Martha Appleton was born in 1620 in Little Waldingfield, co. Suffolk, England. She was baptized on 12 Nov 1620 in Little Waldingfield, co. Suffolk, England. She died on 8 Sep 1659.
iv. Capt. John Appleton was born in 1622 in Little Waldingfield, co. Suffolk, England. He was baptized on 17 Nov 1622 in Little Waldingfield, co. Suffolk, England. He died on 4 Nov 1699 in Ipswich, Essex Co., Massachusetts.
v. Maj. Samuel Appleton Jr..
vi. Sarah Appleton was born about 1627/28 in Reydon, England. Waters says 1629. She died on 22 Apr 1696 in Rowley, Essex Co., Massachusetts. She died on 15 Jul 1714.

Samuel Appleton married second Martha about 1633.